Methods for generating polynucleotides having desired characteristics by iterative selection and recombination - Patent US6117679(A)

Filed: Mar 25, 1996

Published: Sep 12, 2000

Abstract

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.